P-9: A New Morphology Staining Method for Sperm of Some Domestic Animals

نویسندگان

  • DaneshZadeh MT
  • Fakheri R
  • Golmohammadi P
  • Shahverdi AH
چکیده مقاله:

Background: There are some methods for morphology staining of sperm. Although many different and also new methods are used in semen analysis, light microscopy is still used for routine morphological evaluation. The most used of the methods is Papanicolaou staining. This method is routinely used in Andrology laboratory for Human sperm morphology staining. In some domestic animals such as bull, Ram and Goat this method is not efficient for sperm staining. Materials and Methods: In this study Papanicolaou method was altered by deleting Hematoxylin dye and adding Giemsa dye to better showing the parameters of sperm, especially acrosome in sperm of domestic animal. Results: By comparison stained slides under the light microscopy results of the study showed that in some animals such as Goat, Ram and Bull sperm is better stained by this method. Conclusion: According to inpermability of some domestic animals sperm to Papanicolaou dyes and light microscopy comparison we conclued that this method with regards to be fast and inexpensive can use for staining in some animals sperm.

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عنوان ژورنال

دوره 5  شماره Supplement Issue

صفحات  -

تاریخ انتشار 2011-09-01

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